Structural and functional insights into the modulation of the activity of a flax cytokinin oxidase by flax rust effector AvrL567‐A
Identifieur interne : 000289 ( Main/Exploration ); précédent : 000288; suivant : 000290Structural and functional insights into the modulation of the activity of a flax cytokinin oxidase by flax rust effector AvrL567‐A
Auteurs : Li Wan ; Markus Koeck ; Simon J. Williams ; Anthony R. Ashton ; Gregory J. Lawrence ; Hitoshi Sakakibara ; Mikiko Kojima ; Christine Böttcher ; Daniel J. Ericsson ; Adrienne R. Hardham ; David A. Jones ; Jeffrey G. Ellis ; Bostjan Kobe ; Peter N. DoddsSource :
- Molecular Plant Pathology [ 1464-6722 ] ; 2018.
Descripteurs français
- KwdFr :
- Basidiomycota (génétique), Basidiomycota (métabolisme), Basidiomycota (pathogénicité), Liaison aux protéines (MeSH), Lin (microbiologie), Lin (métabolisme), Maladies des plantes (microbiologie), Oxidoreductases (génétique), Oxidoreductases (métabolisme), Protéines fongiques (génétique), Protéines fongiques (métabolisme), Protéines végétales (génétique), Protéines végétales (métabolisme), Techniques de double hybride (MeSH).
- MESH :
- génétique : Basidiomycota, Oxidoreductases, Protéines fongiques, Protéines végétales.
- microbiologie : Lin, Maladies des plantes.
- métabolisme : Basidiomycota, Lin, Oxidoreductases, Protéines fongiques, Protéines végétales.
- pathogénicité : Basidiomycota.
- Liaison aux protéines, Techniques de double hybride.
English descriptors
- KwdEn :
- Basidiomycota (genetics), Basidiomycota (metabolism), Basidiomycota (pathogenicity), Flax (metabolism), Flax (microbiology), Fungal Proteins (genetics), Fungal Proteins (metabolism), Oxidoreductases (genetics), Oxidoreductases (metabolism), Plant Diseases (microbiology), Plant Proteins (genetics), Plant Proteins (metabolism), Protein Binding (MeSH), Two-Hybrid System Techniques (MeSH).
- MESH :
- chemical , genetics : Fungal Proteins, Oxidoreductases, Plant Proteins.
- genetics : Basidiomycota.
- metabolism : Basidiomycota, Flax, Fungal Proteins, Oxidoreductases, Plant Proteins.
- microbiology : Flax, Plant Diseases.
- pathogenicity : Basidiomycota.
- Protein Binding, Two-Hybrid System Techniques.
Abstract
During infection, plant pathogens secrete effector proteins to facilitate colonization. In comparison with our knowledge of bacterial effectors, the current understanding of how fungal effectors function is limited. In this study, we show that the effector AvrL567‐A from the flax rust fungus
Url:
DOI: 10.1111/mpp.12749
PubMed: 30242946
PubMed Central: 6637871
Affiliations:
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Le document en format XML
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Williams</name><affiliation><nlm:aff id="mpp12749-aff-0001"></nlm:aff></affiliation><affiliation><nlm:aff id="mpp12749-aff-0004"></nlm:aff></affiliation></author><author><name sortKey="Ashton, Anthony R" sort="Ashton, Anthony R" uniqKey="Ashton A" first="Anthony R." last="Ashton">Anthony R. Ashton</name><affiliation><nlm:aff id="mpp12749-aff-0003"></nlm:aff></affiliation></author><author><name sortKey="Lawrence, Gregory J" sort="Lawrence, Gregory J" uniqKey="Lawrence G" first="Gregory J." last="Lawrence">Gregory J. Lawrence</name><affiliation><nlm:aff id="mpp12749-aff-0003"></nlm:aff></affiliation></author><author><name sortKey="Sakakibara, Hitoshi" sort="Sakakibara, Hitoshi" uniqKey="Sakakibara H" first="Hitoshi" last="Sakakibara">Hitoshi Sakakibara</name><affiliation><nlm:aff id="mpp12749-aff-0005"></nlm:aff></affiliation></author><author><name sortKey="Kojima, Mikiko" sort="Kojima, Mikiko" uniqKey="Kojima M" first="Mikiko" last="Kojima">Mikiko Kojima</name><affiliation><nlm:aff id="mpp12749-aff-0005"></nlm:aff></affiliation></author><author><name sortKey="Bottcher, Christine" sort="Bottcher, Christine" uniqKey="Bottcher C" first="Christine" last="Böttcher">Christine Böttcher</name><affiliation><nlm:aff id="mpp12749-aff-0006"></nlm:aff></affiliation></author><author><name sortKey="Ericsson, Daniel J" sort="Ericsson, Daniel J" uniqKey="Ericsson D" first="Daniel J." last="Ericsson">Daniel J. Ericsson</name><affiliation><nlm:aff id="mpp12749-aff-0007"></nlm:aff></affiliation></author><author><name sortKey="Hardham, Adrienne R" sort="Hardham, Adrienne R" uniqKey="Hardham A" first="Adrienne R." last="Hardham">Adrienne R. Hardham</name><affiliation><nlm:aff id="mpp12749-aff-0004"></nlm:aff></affiliation></author><author><name sortKey="Jones, David A" sort="Jones, David A" uniqKey="Jones D" first="David A." last="Jones">David A. Jones</name><affiliation><nlm:aff id="mpp12749-aff-0004"></nlm:aff></affiliation></author><author><name sortKey="Ellis, Jeffrey G" sort="Ellis, Jeffrey G" uniqKey="Ellis J" first="Jeffrey G." last="Ellis">Jeffrey G. Ellis</name><affiliation><nlm:aff id="mpp12749-aff-0003"></nlm:aff></affiliation></author><author><name sortKey="Kobe, Bostjan" sort="Kobe, Bostjan" uniqKey="Kobe B" first="Bostjan" last="Kobe">Bostjan Kobe</name><affiliation><nlm:aff id="mpp12749-aff-0001"></nlm:aff></affiliation></author><author><name sortKey="Dodds, Peter N" sort="Dodds, Peter N" uniqKey="Dodds P" first="Peter N." last="Dodds">Peter N. Dodds</name><affiliation><nlm:aff id="mpp12749-aff-0003"></nlm:aff></affiliation></author></analytic><series><title level="j">Molecular Plant Pathology</title><idno type="ISSN">1464-6722</idno><idno type="eISSN">1364-3703</idno><imprint><date when="2018">2018</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Basidiomycota (genetics)</term><term>Basidiomycota (metabolism)</term><term>Basidiomycota (pathogenicity)</term><term>Flax (metabolism)</term><term>Flax (microbiology)</term><term>Fungal Proteins (genetics)</term><term>Fungal Proteins (metabolism)</term><term>Oxidoreductases (genetics)</term><term>Oxidoreductases (metabolism)</term><term>Plant Diseases (microbiology)</term><term>Plant Proteins (genetics)</term><term>Plant Proteins (metabolism)</term><term>Protein Binding (MeSH)</term><term>Two-Hybrid System Techniques (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Basidiomycota (génétique)</term><term>Basidiomycota (métabolisme)</term><term>Basidiomycota (pathogénicité)</term><term>Liaison aux protéines (MeSH)</term><term>Lin (microbiologie)</term><term>Lin (métabolisme)</term><term>Maladies des plantes (microbiologie)</term><term>Oxidoreductases (génétique)</term><term>Oxidoreductases (métabolisme)</term><term>Protéines fongiques (génétique)</term><term>Protéines fongiques (métabolisme)</term><term>Protéines végétales (génétique)</term><term>Protéines végétales (métabolisme)</term><term>Techniques de double hybride (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Fungal Proteins</term><term>Oxidoreductases</term><term>Plant Proteins</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Basidiomycota</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Basidiomycota</term><term>Oxidoreductases</term><term>Protéines fongiques</term><term>Protéines végétales</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Basidiomycota</term><term>Flax</term><term>Fungal Proteins</term><term>Oxidoreductases</term><term>Plant Proteins</term></keywords><keywords scheme="MESH" qualifier="microbiologie" xml:lang="fr"><term>Lin</term><term>Maladies des plantes</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Flax</term><term>Plant Diseases</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Basidiomycota</term><term>Lin</term><term>Oxidoreductases</term><term>Protéines fongiques</term><term>Protéines végétales</term></keywords><keywords scheme="MESH" qualifier="pathogenicity" xml:lang="en"><term>Basidiomycota</term></keywords><keywords scheme="MESH" qualifier="pathogénicité" xml:lang="fr"><term>Basidiomycota</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Protein Binding</term><term>Two-Hybrid System Techniques</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Liaison aux protéines</term><term>Techniques de double hybride</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><title>Summary</title><p>During infection, plant pathogens secrete effector proteins to facilitate colonization. In comparison with our knowledge of bacterial effectors, the current understanding of how fungal effectors function is limited. In this study, we show that the effector AvrL567‐A from the flax rust fungus <italic>Melampsora lini</italic> interacts with a flax cytosolic cytokinin oxidase, LuCKX1.1, using both yeast two‐hybrid and <italic>in planta</italic> bimolecular fluorescence assays. Purified LuCKX1.1 protein shows catalytic activity against both N6‐(Δ2‐isopentenyl)‐adenine (2iP) and <italic>trans</italic>‐zeatin (tZ) substrates. Incubation of LuCKX1.1 with AvrL567‐A results in increased catalytic activity against both substrates. The crystal structure of LuCKX1.1 and docking studies with AvrL567‐A indicate that the AvrL567 binding site involves a flexible surface‐exposed region that surrounds the cytokinin substrate access site, which may explain its effect in modulating LuCKX1.1 activity. Expression of AvrL567‐A in transgenic flax plants gave rise to an epinastic leaf phenotype consistent with hormonal effects, although no difference in overall cytokinin levels was observed. We propose that, during infection, plant pathogens may differentially modify the levels of extracellular and intracellular cytokinins.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct></listBibl></div1></back></TEI><affiliations><list></list><tree><noCountry><name sortKey="Ashton, Anthony R" sort="Ashton, Anthony R" uniqKey="Ashton A" first="Anthony R." last="Ashton">Anthony R. Ashton</name><name sortKey="Bottcher, Christine" sort="Bottcher, Christine" uniqKey="Bottcher C" first="Christine" last="Böttcher">Christine Böttcher</name><name sortKey="Dodds, Peter N" sort="Dodds, Peter N" uniqKey="Dodds P" first="Peter N." last="Dodds">Peter N. Dodds</name><name sortKey="Ellis, Jeffrey G" sort="Ellis, Jeffrey G" uniqKey="Ellis J" first="Jeffrey G." last="Ellis">Jeffrey G. Ellis</name><name sortKey="Ericsson, Daniel J" sort="Ericsson, Daniel J" uniqKey="Ericsson D" first="Daniel J." last="Ericsson">Daniel J. Ericsson</name><name sortKey="Hardham, Adrienne R" sort="Hardham, Adrienne R" uniqKey="Hardham A" first="Adrienne R." last="Hardham">Adrienne R. Hardham</name><name sortKey="Jones, David A" sort="Jones, David A" uniqKey="Jones D" first="David A." last="Jones">David A. Jones</name><name sortKey="Kobe, Bostjan" sort="Kobe, Bostjan" uniqKey="Kobe B" first="Bostjan" last="Kobe">Bostjan Kobe</name><name sortKey="Koeck, Markus" sort="Koeck, Markus" uniqKey="Koeck M" first="Markus" last="Koeck">Markus Koeck</name><name sortKey="Kojima, Mikiko" sort="Kojima, Mikiko" uniqKey="Kojima M" first="Mikiko" last="Kojima">Mikiko Kojima</name><name sortKey="Lawrence, Gregory J" sort="Lawrence, Gregory J" uniqKey="Lawrence G" first="Gregory J." last="Lawrence">Gregory J. Lawrence</name><name sortKey="Sakakibara, Hitoshi" sort="Sakakibara, Hitoshi" uniqKey="Sakakibara H" first="Hitoshi" last="Sakakibara">Hitoshi Sakakibara</name><name sortKey="Wan, Li" sort="Wan, Li" uniqKey="Wan L" first="Li" last="Wan">Li Wan</name><name sortKey="Williams, Simon J" sort="Williams, Simon J" uniqKey="Williams S" first="Simon J." last="Williams">Simon J. Williams</name></noCountry></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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